LAMP1 anticorps

N° du produit ABIN967382

L’anticorps Rat Monoclonal anti-LAMP1 a été validé pour WB, IP, IHC (fro), ICS et IAC. Il convient pour détecter LAMP1 dans des échantillons de Souris. Il y a 4+ publications disponibles.

Aperçu rapide pour LAMP1 anticorps (ABIN967382)

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp LAMP1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoaffinity Chromatography (IAC)

Clone: 1D4B

Détail du produit anti-LAMP1 anticorps

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Plasma membrane fraction of mouse embryo NIH 3T3 cell line

Isotype: IgG2a kappa

Information d'application

Indications d'application: This antibody conjugate has been tested by intracellular immunofluorescent staining ( Less or equal than 1 µg/million cells, using the Cytofix/Cytoperm™ Kit) with flow cytometric analysis to assure specificity and reactivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C.

Références pour anticorps anti-LAMP1 (ABIN967382)

Rohrer, Schweizer, Russell, Kornfeld: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." dans: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, (1996) (PubMed).

Arterburn, Earles, August: "The disulfide structure of mouse lysosome-associated membrane protein 1." dans: The Journal of biological chemistry, Vol. 265, Issue 13, pp. 7419-23, (1990) (PubMed).

Chen, Chen, DSouza, Murphy, August: "Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2." dans: Biochemical Society symposium, Vol. 51, pp. 97-112, (1987) (PubMed).

Chen, Pan, DSouza, August: "Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells." dans: Archives of biochemistry and biophysics, Vol. 239, Issue 2, pp. 574-86, (1985) (PubMed).

Détails sur LAMP1

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Autre désignation: CD107a

Sujet: The 1D4B antibody recognizes CD107a (Lysosome-Associated Membrane Protein 1, LAMP-1), one of the two major glycoproteins in lysosome membranes which are useful markers to distinguish lysosomes from other organelles. Mouse CD107a consists of a 40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoprotein of 110-140 kDa. It is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice. Several lines of evidence suggest that cell-surface mouse and human LAMP-1 glycoproteins, the expression of which may correlate with malignant transformation, participate in intercellular adhesion and adhesion to extracellular matrix.
Synonyms: LAMP-1

Pathways: Autophagy